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hep 2 cells  (ATCC)


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    ATCC hep 2 cells
    Hep 2 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3832 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hep+2+cells+ccl+23/HEp-2/pm42319946-273-5-8
    Average 99 stars, based on 3832 article reviews
    hep 2 cells - by Bioz Stars, 2026-09
    99/100 stars

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    Cell Culture:

    Article Title: The Interferon Type I/III Response to Respiratory Syncytial Virus Infection in Airway Epithelial Cells Can Be Attenuated or Amplified by Antiviral Treatment
    Article Snippet: .. HEp-2 cells (CCL-23) and A549 cells (CCL-185) were obtained from American Type Culture Collection and cultured in minimal essential medium (MEM) (1×) and GlutaMAX with Earle's salts (Life Technologies) or F12K nutrient mixture (Kaighn's modification) with l -glutamine (Life Technologies), respectively, supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; HyClone), 10 units/ml penicillin, and 10 μg/ml streptomycin. .. The MT4 cell line was obtained from the NIH AIDS Research and Reference Reagent Program (Germantown, MD) and cultured in RPMI 1640 medium (Irvine Scientific) supplemented with 10% FBS, 100 units/ml penicillin, 100 units/ml streptomycin, and 2 mM l -glutamine.

    Modification:

    Article Title: The Interferon Type I/III Response to Respiratory Syncytial Virus Infection in Airway Epithelial Cells Can Be Attenuated or Amplified by Antiviral Treatment
    Article Snippet: .. HEp-2 cells (CCL-23) and A549 cells (CCL-185) were obtained from American Type Culture Collection and cultured in minimal essential medium (MEM) (1×) and GlutaMAX with Earle's salts (Life Technologies) or F12K nutrient mixture (Kaighn's modification) with l -glutamine (Life Technologies), respectively, supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; HyClone), 10 units/ml penicillin, and 10 μg/ml streptomycin. .. The MT4 cell line was obtained from the NIH AIDS Research and Reference Reagent Program (Germantown, MD) and cultured in RPMI 1640 medium (Irvine Scientific) supplemented with 10% FBS, 100 units/ml penicillin, 100 units/ml streptomycin, and 2 mM l -glutamine.

    Article Title: Modulation of Ricin Intoxication by the Autophagy Inhibitor EACC
    Article Snippet: .. HEp-2 cells (CCL-23) and HeLa cells were from ATCC (Manassas, VA, USA) and both cell types were grown in Dulbecco’s Modified Eagle Medium (DMEM, D0819, Sigma-Aldrich, Saint Louis, MO, USA), with 10% fetal serum (FBS, F7524, Sigma-Aldrich, Irvine, United Kingdom). .. PC-3 cells (CRL-1435) were from ATCC (Manassas, VA, USA) and were grown in DMEM/F-12 (1:1) (31331-093, Thermo-Fisher Scientific) with 7% FBS.



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    Optimization of cell-related conditions for the RSV mRNA potency assay. ( A ) Dose–response curves of RSV mRNA transfection in different cell lines (293T, Vero, <t>HEp-2,</t> HepG2, and A549). Cells were transfected with increasing amounts of RSV mRNA (0.0002–0.48 µg/well), and RSV prefusion F protein expression was measured by ELISA as OD 450 –OD 630 . The corresponding EC 50 and slope values are summarized in the inset table. ( B ) Effect of HepG2 cell seeding density on RSV prefusion F protein expression. HepG2 cells were seeded at 0.5 × 10 5 , 1.0 × 10 5 , or 2.0 × 10 5 cells/well and transfected with increasing amounts of RSV mRNA (0.0002–0.48 µg/well). Protein expression was measured by ELISA as OD 450 –OD 630 .
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    Optimization of cell-related conditions for the RSV mRNA potency assay. ( A ) Dose–response curves of RSV mRNA transfection in different cell lines (293T, Vero, <t>HEp-2,</t> HepG2, and A549). Cells were transfected with increasing amounts of RSV mRNA (0.0002–0.48 µg/well), and RSV prefusion F protein expression was measured by ELISA as OD 450 –OD 630 . The corresponding EC 50 and slope values are summarized in the inset table. ( B ) Effect of HepG2 cell seeding density on RSV prefusion F protein expression. HepG2 cells were seeded at 0.5 × 10 5 , 1.0 × 10 5 , or 2.0 × 10 5 cells/well and transfected with increasing amounts of RSV mRNA (0.0002–0.48 µg/well). Protein expression was measured by ELISA as OD 450 –OD 630 .
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    Optimization of cell-related conditions for the RSV mRNA potency assay. ( A ) Dose–response curves of RSV mRNA transfection in different cell lines (293T, Vero, HEp-2, HepG2, and A549). Cells were transfected with increasing amounts of RSV mRNA (0.0002–0.48 µg/well), and RSV prefusion F protein expression was measured by ELISA as OD 450 –OD 630 . The corresponding EC 50 and slope values are summarized in the inset table. ( B ) Effect of HepG2 cell seeding density on RSV prefusion F protein expression. HepG2 cells were seeded at 0.5 × 10 5 , 1.0 × 10 5 , or 2.0 × 10 5 cells/well and transfected with increasing amounts of RSV mRNA (0.0002–0.48 µg/well). Protein expression was measured by ELISA as OD 450 –OD 630 .

    Journal: Vaccines

    Article Title: Establishment and Validation of a Cell-Based Relative Potency Method for Respiratory Syncytial Virus mRNA Vaccine Drug Substance

    doi: 10.3390/vaccines14050401

    Figure Lengend Snippet: Optimization of cell-related conditions for the RSV mRNA potency assay. ( A ) Dose–response curves of RSV mRNA transfection in different cell lines (293T, Vero, HEp-2, HepG2, and A549). Cells were transfected with increasing amounts of RSV mRNA (0.0002–0.48 µg/well), and RSV prefusion F protein expression was measured by ELISA as OD 450 –OD 630 . The corresponding EC 50 and slope values are summarized in the inset table. ( B ) Effect of HepG2 cell seeding density on RSV prefusion F protein expression. HepG2 cells were seeded at 0.5 × 10 5 , 1.0 × 10 5 , or 2.0 × 10 5 cells/well and transfected with increasing amounts of RSV mRNA (0.0002–0.48 µg/well). Protein expression was measured by ELISA as OD 450 –OD 630 .

    Article Snippet: Cell lines used for mRNA transfection included the human liver carcinoma cell line HepG2 (American Type Culture Collection, ATCC, Manassas, VA, USA), human non-small cell lung carcinoma cell line A549 (ATCC), African green monkey kidney epithelial cell line Vero (ATCC), human epidermoid carcinoma type 2 cell line Hep-2 (ATCC), and human embryonic kidney cell line 293T (China Center for Type Culture Collection, CCTCC, Wuhan, China).

    Techniques: Potency Assay, Transfection, Expressing, Enzyme-linked Immunosorbent Assay